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KMID : 1123920020160010191
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 1 p.191 ~ p.196
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Puerariae radix increases Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Dentate Gyrus of Rats
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Jang Mi-Hyeon
Lee Taeck-Hyun
Shin Min-Chul
Lim Baek-Vin
Kim Hyun-Bae
Lim Sabina
Kim Jin-Woo
Lee Cheong-Yeal
Kim E-Hwa
Kim Chang-Ju
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Abstract
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Traditionally, aqueous extracts of Puerariae radix had been used for the treatment of alcohol-related problems. In the present study, the effect of Puerariae radix on cell proliferation and expression of nitric oxide synthase (NOS) in the dentate gyrus of alcohol-intoxicated rats were investigated via 5-bromo-2’ -deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, respectively. Sprague-Dawley rats weighing 150 ± 10 g were divided into four groups: the control group, the Puerarias radix-treated group, the alcohol-treated group, and the alcohol- and Puerariae radix-treated group. The numbers of both BrdU-positive and NADPH-d-positive cells in the dentate gyrus were inhibited significantly by alcohol administration, while Puerariae radix treatment was shown to increase those numbers. In this study, it was revealed that Puerariae radix possesses protective effect against alcohol-induced suppressed new cell formation and NOS expression in the dentate gyrus. Based on the results, it is possible that NO, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol.
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KEYWORD
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Puerariae radix, alcohol, dentate gyrus, 5-bromo-2’ -deoxyuridine, nicotinamide adenine dinudeotide phosphate-diaphorase
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